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Figure 3

From: Contribution of extrahepatic small cells resembling small hepatocyte-like progenitor cells to liver mass maintenance in transplantation model of retrorsine-pretreated liver

Figure 3

Pathological features of retrorsine-pretreated liver after LT and GFP positive hepatocyte-like cells. (A) and (B) Eight weeks after whole transplantation of retrorsine-pretreated liver shows nodules of SHPCs. (A) Hematoxylin Eosin staining after 10% formalin fixation. (B) GFP staining and Hematoxylin staining after 2% paraformalin fixation. The cell structure is well preserved. The surrounding hepatocytes are larger than normal. (C) and (D) Four weeks after transplantation of 50% retrorsine pretreated liver shows abnormally large hepatocytes and SHPCs. (C) IHC staining for GFP. Observation of serial section confirmed the fragment of GFP positive cell is a part of the main cell. Lower part of the picture is SHPCs. (D) Higher magnification of (C). Bar is 50 um.

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