Table 1 Experimental design and results of the four-factor-two-level factorial design together with the predicted yields from the pure first-order model
Factorsa | X1 (g/L) | X2 (g/L) | X3 (g/L) | X4 (g/L) | Observed MCA (SU/mL) | Predicted MCA (SU/mL) |
|---|---|---|---|---|---|---|
Trial no. | ||||||
1 | 10 | 2 | 1 | 8 | 28.57 | 84.1162 |
2 | 50 | 2 | 1 | 8 | 533.33 | 639.2012 |
3 | 10 | 8 | 1 | 8 | 12.31 | −31.3188 |
4 | 50 | 8 | 1 | 8 | 505.26 | 523.7662 |
5 | 10 | 2 | 3 | 8 | 40 | 86.6212 |
6 | 50 | 2 | 3 | 8 | 685.71 | 641.7063 |
7 | 10 | 8 | 3 | 8 | 25.8 | −28.8138 |
8 | 50 | 8 | 3 | 8 | 417.39 | 526.2712 |
9 | 10 | 2 | 1 | 16 | 64 | 142.4388 |
10 | 50 | 2 | 1 | 16 | 738.46 | 697.5237 |
11 | 10 | 8 | 1 | 16 | 25 | 27.0037 |
12 | 50 | 8 | 1 | 16 | 564.71 | 582.0888 |
13 | 10 | 2 | 3 | 16 | 53.33 | 144.9438 |
14 | 50 | 2 | 3 | 16 | 800 | 700.0288 |
15 | 10 | 8 | 3 | 16 | 12.31 | 29.5088 |
16 | 50 | 8 | 3 | 16 | 457.14 | 584.5938 |
b17(C) | 30 | 5 | 2 | 12 | 533.33 | 334.355 |
b18(C) | 30 | 5 | 2 | 12 | 521.74 | 334.355 |