Figure 2

Kinetics of EV71 replication in HT29 cells. Confluent HT29 cells were infected with or without EV71 (MOI of 1). Total intracellular RNA were harvested at various time points, converted to cDNA and measured by quantitative real time polymerase chain reaction (qRT-PCR) with primers specific to viral VP1. (n = 3, * = p values of < 0.05) As observed, there was an increase in viral copy number through increasingly time points.