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Figure 4

From: A simple set of validation steps identifies and removes false results in a sandwich enzyme-linked immunosorbent assay caused by anti-animal IgG antibodies in plasma from arthritis patients

Figure 4

Additional testing of the ELISA system. (A) Testing the ELISA system for variability. Calculated IL-24 concentrations in the positive control sample analyzed in nine different experiments. (B) Testing the ELISA system for stability of the analyte. Calculated IL-24 concentrations in the positive control sample after storage under different conditions or after a variable number of freeze/thaw cycles (f/t). (C) Testing the ELISA system for effect of the sample anticoagulant used. Calculated IL-24 concentrations in eight paired plasma samples from patients with arthritis stabilized with either EDTA or heparin or serum samples. (D) Testing the ELISA system for effect on the standard curve of adding immunoglobulins to the assay diluent. OD signal from samples of rh IL-24 diluted in assay buffer without immunoglobulin and from samples of rh IL-24 diluted in assay buffer with immunoglobulin.

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