Assay | Inoculum concentration (cells/mL) | Rocking mechanism | Rocking speed (rpm) | Volume at inoculation | Culture vessel |
---|---|---|---|---|---|
CY1 | 2×105 | Pulse followed by continuousa | 60 | Total | Vented conical tube |
CY2 | 2×105 | Continuous | 60 | Total | Vented conical tube |
CY3 | 2×105 | Pulse followed by continuousa | 40 | Total | Vented conical tube |
CY4 | 2×105 | Continuous | 40 | Total | Vented conical tube |
CY5 | 2×105 | Pulse followed by continuousa | 60 | Half | Vented conical tube |
CY6 | 4×105 | Pulse followed by continuousa | 60 | Total | Vented conical tube |
CY7 | 4×105 | Continuous | 60 | Total | Vented conical tube |
CYS | 4x105 | Pulse followed by continuousa | 60 | Total | Shake flask |