Step | Volume (mL) | Total protein concentration (mg/mL) | Purity (%)a | Amount of LALF32-51-E7 (mg) | Purification fold | Step yield (%) | Overall yield (%) |
---|---|---|---|---|---|---|---|
Initial crude extract | 30 | 5.85 | 18 | 31.59 | 1 | 100 | 100 |
Insoluble fraction after cell disruption | 30 | 2.33 | 32 | 22.37 | 1.7 | 71 | 71 |
Solubilization | 15 | 3.07 | 45 | 20.72 | 1.4 | 93 | 66 |
IMAC elution | 25 | 0.75 | 94 | 17.63 | 2 | 85 | 56 |
Desalting | 35 | 0.5 | 94 | 16.45 | 1 | 93 | 52 |