Chitinolytic assay of indigenous Trichoderma isolates collected from different geographical locations of Chhattisgarh in Central India

Agrawal, Toshy; Kotasthane, Anil S

doi:10.1186/2193-1801-1-73

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Table 2 Spectrophotometric determination of Chitinolytic and Exochitinase activity of Trichoderma isolates in media supplemented with colloidal chitin derived from Rhizoctonia cell wall (RCW) and Commercial chitin (Cc)

From: Chitinolytic assay of indigenous Trichoderma isolates collected from different geographical locations of Chhattisgarh in Central India

Isolate No.	NAGA conc. (mg/ml)		Volume activity (U/ml X 10^-3)
	RCW	Cc	RCW	Cc
GROUP A *(T. harzianum / viride)*
1	57.67	54.33	4.26	4.54
4	51	111	4.26	17.89
6	41	41	6.82	6.76
10	37.67	37.67	1.99	6.65
14	51	47.67	3.69	7.04
20	74.33	91	33.17	3.41
25	87.67	61	4.66	5.62
32	74.33	104.33	34.02	5.85
68	44.33	64.33	1.25	2.95
73	127.67	77.67	2.84	3.12
120	91	57.67	2.10	5.57
207	67.67	54.33	2.89	0.63
211	64.33	71	1.31	1.76
217	64.33	37.67	9.09	1.48
Th1	74.33	84.33	0.63	1.19
Tv(t)	71	54.33	4.89	7.1
Mean	67.46	65.58	7.37	5.09
Mean	(5.59)	(5.66)	(2.62)	(1.02)
P	0.4837^ns		0.0004^s
Group B ( *T. citirinoviride* )
24	61	57.67	13.52	20.51
27	47.67	61	1.36	2.73
28	57.67	54.33	9.26	27.83
29	67.67	141	5.96	12.49
43	57.67	54.33	14.37	3.41
Mean	58.34	73.67	8.89	13.39
Mean	(3.23)	(16.88)	(2.42)	(4.86)
P	0.0037^s		0.102^ns
Group C ( *T. aureoviride* )
12	81	54.33	0.17	18.80
38	81	114.33	22.44	1.76
48	67.67	51	35.16	27.21
55	81	61	2.49	4.88
62	71	47.67	33.06	18.69
98	101	61	17.44	1.25
107	84.33	41	8.69	8.80
202	87.67	107.67	35.78	22.15
249	81	51	13.18	0.85
297	77.67	51	3.35	21.24
231a	87.67	64.33	15.90	27.38
Gv	74.33	61	12.89	24.77
Mean	81.28	63.78	16.71	14.82
Mean	(2.51)	(6.66)	(3.64)	(3.04)
P	0.0015^s		0.2799^ns
Group D ( *T. virens* )
85	61	327.67	9.88	19.54
93	64.33	154.33	30.33	14.26
99	64.33	234.33	0.68	12.55
152	77.67	71	34.76	19.09
173	71	77.67	9.43	19.77
226	64.33	107.67	23.57	15.62
261	71	77.67	30.16	32.60
326	61	94.33	26.18	19.82
145b1	174.33	64.33	22.38	23.69
Mean	78.78	134.33	20.82	19.66
P	0.0091^s		0.0282^s
Group E ( *Isolates of Sec.L ongibrachiatum* )
Cb4	84.33	81	20.85	14.88
2	91	61	5.62	16.36
46	84.33	54.33	23.69	10.51
53	84.33	51	7.61	14.77
75	81	57.67	19.31	13.69
97	74.33	64.33	2.73	1.48
102	84.33	61	35.5	15.68
Mean	83.38	61.48	16.47	12.48
Mean	(1.89)	(3.67)	(4.44)	(1.96)
P	0.065^ns		0.0342^s
Group F ( *Isolates of Sec. Pachybasium* )
8	74.33	94.33	4.71	13.41
91	47.67	51	0.45	0.91
106	67.67	54.33	1.54	2.61
111	77.67	74.33	25.28	7.78
114	71	101	1.82	8.41
204	114.33	61	12.16	5.79
238	87.67	57.67	1.02	7.89
243	91	51	24.31	13.41
332	87.67	57.67	0.34	1.42
125b	94.33	61	5.79	9.09
233b	77.67	57.67	5.62	21.87
40(1)b	74.33	47.67	13.92	9.71
Mean	80.45	64.06	8.08	8.53
Mean	(4.75)	(4.95)	(2.56)	(1.69)
P	0.4463^ns		0.0886^ns

Figures in parenthesis represent standard error.
^s =significant(P-value<0.05); ^ns = non-significant (P-value>0.05).

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