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Figure 3 | SpringerPlus

Figure 3

From: Inhibition of cancer cell proliferation by 5-fluoro-2'-deoxycytidine, a DNA methylation inhibitor, through activation of DNA damage response pathway

Figure 3

FCdR induced alteration of global gene expression pattern. 0.5 μM FCdR or 375 μM 5-Fu were used to treat HCT116 cells for 24 h. Total RNA was used for gene expression pattern analysis by high throughput sequencing. A. The number of differentially expressed genes was calculated. FCdR caused changes in expression of 1165 genes, out of which 757 were up-regulated and 408 were down-regulated. 5-Fu caused changes in expression of 3295 genes, out of which 1103 were up-regulated, and 2193 were down-regulated. FCdR-treated cells have a much higher percentage of up-regulated genes. B. Differentially expressed genes involved in top 6 pathways (p53 signaling pathway, colorectal cancer, nucleotide excision repair, DNA replication, cell cycle, pathways in cancer) were subjected to cluster analysis. C. The change in expression of 45 genes was confirmed by real time PCR. (* p value < 0.05; ** p value < 0.01).

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