Figure 3

CLTC expression is increased in the cells treated with albumin-associated lipids and with petroselinic acid. MALME-3M and MCF-7 cells were treated with albumin-associated lipids and petroselinic acid (100 μg/ml) for 96 hours. Cells were lysed, separated in a 4–12% Bis-Tris gel and probed for western blotting with antibodies against CLTC and Actin (A). The western blot band intensity corresponding to CLTC was normalized to the corresponding Actin band for each sample (B). The average values from densitometry analyses of three separate experiments are shown. *P <0.05 compared to each control.