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Table 1 Primers used in regular and Nested PCR detection of S. Paratyphi A

From: Purification and antigenic detection of O-specific polysaccharides of Salmonella enterica serovar Paratyphi A isolate from Pakistan: an emerging threat

PCR

Primers

Gene

Sequence (5′–3′)

Amplicon size (bp)

Regular

fliC-s

fliCa-as

fliC-a

AATCAACAACAACCTGCAGCG

TAGTGCTTAATGTAGCCGAAGG

329

Nested

N-fliC-s

N-fliCa-as

fliC-a

GCAGCGTGTGCGTGAACTGGCGG

GACTTCGCTCTTCACATCATAT

289